Isolation and Characterization of the Thymus-Brain Antigen
نویسنده
چکیده
1. The human thymus-brain antigen, which corresponds to the murine (mouse or rat) Thy1 antigen complex, was isolated from brain after solubilization in deoxycholate by gel-permeation chromatography, wheat-germ-lectin affinity chromatography and ionexchange chromatography. 2. The isolated antigen is a glycoprotein displaying an apparent molecular weight of 26000-29000 in sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. 3. No antigen activity was found with the lipid fraction from human brain. 4. The protein has a tendency for spontaneous self-association (dimerization), leading to aggregates resistant to dissociating and reducing agents on prolonged storage. 5. The antigen is microheterogeneous with respect to size, charge (approximate isoelectric points of the monomer 7.7, 7.0 and 6.5) and to lectin-binding affinity. 6. The antigen can be reconstituted to protein-lipid vesicles. The antigen activity of solubilized antigen is strongly increased by reconstitution and that of membranes decreased by solubilization with detergent.
منابع مشابه
Isolation and characterization of a defective measles virus from brain biopsies of three patients in Iran with subacute sclerosing panencephalitis
متن کامل
Production and Characterization of Monoclonal Antibodies Recognizing a Common 57-kDa Antigen of Leishmania Species
Background: The therapy of leishmania infection is difficult and each year 1.5 million new cases of cutaneous leishmaniasis and 500,000 new cases of visceral leishmaniasis are estimated, therefore, there is a need for an effective vaccine. Monoclonal antibody (mAb) is one of the suitable methods for isolation and purification of leishmania antigens. In this report, we produced several mAb aga...
متن کاملIsolation of myeloid dendritic cells and epithelial cells from human thymus.
In this protocol we provide a method to isolate dendritic cells (DC) and epithelial cells (TEC) from the human thymus. DC and TEC are the major antigen presenting cell (APC) types found in a normal thymus and it is well established that they play distinct roles during thymic selection. These cells are localized in distinct microenvironments in the thymus and each APC type makes up only a minor ...
متن کاملIsolation, identification and biodiversity of endophytic fungi o Thymus
Endophytes are considered as an important source of bioactive natural products because thesemicroorganisms are able to occupy unique biological niches and grow in non-ordinaryenvironments. Therefore, endophytic fungi of medicinal plants can be developed for medicinalapplications. On the other hand, medicinal properties of plant can be ascribed to its endophyticfungi. Thymus sp. is widely used a...
متن کاملIsolation and purification of Echinococcus granulosus antigen B from hydatid cyst fluid using three different methods
Hydatid cyst, the larval stage of cestodes Echinococcus spp., is recognized as a zoonotic infection in the world. The World Health Organization (WHO) has recently classified echinococcosis in a group of neglected tropical diseases.The prevalence of Echinococcus granulosus infection is high in Iran due to the presence of various intermediate hosts in this country. Considering the rising trend of...
متن کامل